Catalog Number - 85018-25P

The 85018‑25P Cogent RP C18 ABX™ HPLC Column is engineered for maximum separation and full scalability, delivering robust, reproducible performance across extreme mobile phase conditions (pH 2–12).

Constructed with a bulky, specially end‑capped C18 phase bonded to high‑purity silica, this column minimizes secondary interactions while maintaining excellent peak shape for acidic, basic, hydrophobic, and neutral analytes, including those with very high or very low pKa values.

✅ Ideal as a USP L1 alternate for demanding acid/base methods

250 mm Length – Absolute Maximum Separation & Highest Purity

• Highest resolving power in the 10 µm ABX analytical range

• Maximizes peak capacity for highly complex mixtures

• Ideal for:

  • closely related compounds

  • trace impurities and difficult separations

• Provides extended retention and superior resolution

• Preferred for final method validation and process‑level separations

✅ Selected when shorter columns cannot achieve required purity or separation quality

4.6 mm ID – Analytical to Process‑Scale Bridge Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development and optimization

  • preparative and process scale‑up workflows

  • final analytical validation

Typical Flow Rate Range

• ~0.8–1.5 mL/min (supports higher flow rates due to 10 µm particles)

✅ Enables direct transfer from analytical development to preparative and process chromatography

10 µm Particle – Process‑Scale & High‑Flow Performance

• Designed for:

  • High flow rates

  • Low backpressure operation

  • Maximum durability under heavy sample loads

✅ Ideal for:

• Preparative method development

• Scale‑up and process chromatography workflows

• Continuous or high‑throughput operations

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Stable under:

  • Strongly acidic mobile phases

  • Strongly basic mobile phases

• Suitable for:

  • high‑pKa and low‑pKa analytes

✅ Enables separations not achievable with conventional C18 columns

Why Choose RP C18 ABX™ Columns (10 µm)

Extreme pH Ruggedness

• Stable across full pH spectrum (2–12)

• Ideal for aggressive and stability‑indicating methods

Process‑Scale Readiness

• Designed for direct scalability from analytical to production workflows

• Enables reliable method transfer across column dimensions

Low Backpressure & High Throughput

• Supports higher flow rates with reduced system strain

• Compatible with standard HPLC systems and scale‑up equipment

✅ Preferred for process development, purification, and large‑scale separations

Application Performance Advantages

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

• Enables:

  • High‑purity separations in complex matrices

  • Reliable performance in scale‑up environments

✅ Ideal for final purification workflows and process development

System & Sample Considerations

• Compatible with standard HPLC systems

• Optimized for high‑flow and preparative‑leaning workflows

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column fouling

• Maintain maximum performance and long‑term reproducibility

Typical Applications

• Preparative method development and scale‑up

• Process chromatography and purification

• HPLC analytical separations

• Acidic and basic compound analysis

• Pharmaceutical process development

• Stability‑indicating and impurity profiling methods

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for maximum separation and process‑scale workflows

• Optimize flow rate for throughput, resolution, and system limits